Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs. (April 2016)

• Record Type: Journal Article
• Authors: Di Mauro, Vittoria; Iafisco, Michele; Salvarani, Nicolò; Vacchiano, Marco; Carullo, Pierluigi; Ramírez-Rodríguez, Gloria Belén; Patrício, Tatiana; Tampieri, Anna; Miragoli, Michele; Catalucci, Daniele
• Is Part Of: Nanomedicine. Volume 11:Number 8(2016)
• Page Start: 891
• Page End: 906
• Publication Date: 2016-04
• Subjects: calcium phosphate nanoparticles -- cardiomyocytes -- drug-delivery systems -- microRNA -- nanomedicine; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.16.26
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart.Materials & methods: CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated.Results: CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo .Conclusion: CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.