Multilayer engineered nanoliposomes as a novel tool for oral delivery of lipopeptide-based vaccines against group A Streptococcus. (May 2016)

• Record Type: Journal Article
• Authors: Marasini, Nirmal; Giddam, Ashwini K; Ghaffar, Khairunnisa A; Batzloff, Michael R; Good, Michael F; Skwarczynski, Mariusz; Toth, Istvan
• Is Part Of: Nanomedicine. Volume 11:Number 10(2016)
• Page Start: 1223
• Page End: 1236
• Publication Date: 2016-05
• Subjects: group A Streptococcus -- lipid-core peptide -- liposomes -- mucosal immunology -- nanoparticles -- oral delivery -- peptides -- vaccines; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.16.36
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To develop an oral nanovaccine delivery system for lipopeptide-based vaccine candidate against group A Streptococcus .Materials & methods: Lipid-core peptide-1-loaded nanoliposomes were prepared as a template and coated with opposite-charged polyelectrolytes to produce particles with size <200 nm. Efficacy of this oral nanovaccine delivery system was evaluated in mice model.Results: Polymer-coated liposomes produced significantly higher antigen-specific mucosal IgA and systemic IgG titers in comparison to vaccine formulated with a strong mucosal adjuvant upon oral immunization in mice. Moreover, high levels of systemic antibody titers were retained even at day 185 postprimary immunization.Conclusion: Efficient oral delivery platform for lipopeptide-based vaccines has been developed.