HLA-G expression and regulation during Pseudomonas aeruginosa infection in cystic fibrosis patients. (March 2016)

• Record Type: Journal Article
• Authors: Rizzo, Roberta; Bergamini, Gabriella; Bortolotti, Daria; Leal, Teresinha; D'Orazio, Ciro; Pintani, Emily; Melchiorri, Loredana; Zavatti, Eleonora; Assael, Baroukh M; Sorio, Claudio; Melotti, Paola
• Is Part Of: Future microbiology. Volume 11:Number 3(2016:Mar.)
• Page Start: 363
• Page End: 373
• Publication Date: 2016-03
• Subjects: cystic fibrosis -- HLA-G -- Pseudomonas aeruginosa; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb.15.143
• Languages: English
• ISSNs: 1746-0913

Abstract

Background: Deregulated immune response fails to control biofilm-forming bacteria, as Pseudomonas aeruginosa, in the lungs of cystic fibrosis (CF) patients. HLA-G is an immune-modulatory molecule involved in respiratory diseases and infections.Materials & methods: HLA-G mRNA and protein were analyzed in plasma and exhaled breath condensate from CF patients undergoing intravenous antibiotic treatment, CF cell line and murine model.Results: Therapy normalizes HLA-G plasmatic in CF patients suggesting a systemic anti-inflammatory role while in CF airway system, higher expression of HLA-G is associated with P. aeruginosa infection. CF cell line and murine model expressed higher HLA-G molecules in the presence of P. aeruginosa .Conclusion: Plasmatic and lung HLA-G expression suggest a role in reducing systemic inflammation and supporting P. aeruginosa infection.