Microstructural changes enhance oxygen transport in tomato (Solanum lycopersicum) fruit during maturation and ripening. Issue 5 (25th September 2021)

• Record Type: Journal Article
• Authors: Xiao, Hui; Piovesan, Agnese; Pols, Suzane; Verboven, Pieter; Nicolaï, Bart
• Is Part Of: New phytologist. Volume 232:Issue 5(2021)
• Page Start: 2043
• Page End: 2056
• Publication Date: 2021-09-25
• Subjects: cell -- hypoxia -- intercellular pore -- maturation and ripening -- microstructure -- oxygen diffusivity -- tomato -- X‐ray micro‐computed tomography; Botany -- Periodicals; 580
• DOI: 10.1111/nph.17712
• Languages: English
• ISSNs: 0028-646X

Abstract

Summary: Climacteric ripening of tomato fruit is initiated by a characteristic surge of the production rate of ethylene, accompanied by an increase in respiration rate. As both activities consume O2 and produce CO2, gas concentration gradients develop in the fruit that cause diffusive transport. This may, in turn, affect respiration and ethylene biosynthesis. Gas diffusion in fruit depends on the amount and connectivity of cells and intercellular spaces in 3D. We investigated micromorphological changes in different tomato tissues during development and ripening by visualizing cells and pores based on high‐resolution micro‐computed tomography, and computed effective O2 diffusivity coefficients based on microstructural features of the tissues. We demonstrated that mesocarp and septa tissues have larger cells but small and more disconnected pores than the placenta and columella, resulting in relatively lower effective O2 diffusivity coefficients. Cell disintegration occurred in the mesocarp and septa during ripening, indicating lysigenous air pore formation and resulting in a gradual increase of the effective O2 diffusivity. The results suggest that hypoxic conditions caused by the increasing size and, hence, diffusion resistance of the growing fruit may induce an increase of tissue porosity that results in a greatly enhanced O2 diffusivity and, thus, helps to alleviate them.