An impedimetric approach for COVID-19 detection. Issue 1 (3rd December 2021)

• Record Type: Journal Article
• Authors: Tepeli Büyüksünetçi, Yudum; Çitil, Burak Ekrem; Anık, Ülkü
• Is Part Of: Analyst. Volume 147:Issue 1(2022)
• Page Start: 130
• Page End: 138
• Publication Date: 2021-12-03
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an01718g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : An impedimetric biosensor that was based on SARS-CoV-2 infection mechanism was developed. Abstract : In this study, an electrochemical approach for the determination of coronavirus disease (COVID-19) was developed. The biosensor system relied on the spike protein (S-protein) based infection mechanism of the virus and included separate interactions of receptors like angiotensin-converting enzyme 2 (ACE2) and CD147. After the optimization of experimental parameters, the analytical characteristics of both receptors ACE2 and CD147 were investigated. For ACE2 receptor, the linear detection ranges of the S-protein were found in the range of 700 ng mL −1 to 1500 ng mL −1 and from 1500 ng mL −1 to 7000 ng mL −1 with a limit of detection (LOD) value of 299.30 ng mL −1 . Meanwhile, for CD147 receptor the linear range was in the range of 500 ng mL −1 to 5000 ng mL −1 with a LOD value of 38.99 ng mL −1 . After the examination of analytical characteristics, the developed electrochemical approach was applied for severe acute respiratory syndrome coronavirus 2 samples and the obtained results were validated with real time polymerase chain reaction method.