Automodification of PARP and fatty acid-based membrane lipidome as a promising integrated biomarker panel in molecular medicine. (March 2016)

• Record Type: Journal Article
• Authors: Bianchi, Anna Rita; Ferreri, Carla; Ruggiero, Simona; Deplano, Simone; Sunda, Valentina; Galloro, Giuseppe; Formisano, Cesare; Faraone Mennella, Maria Rosaria
• Is Part Of: Biomarkers in medicine. Volume 10:Number 3(2016)
• Page Start: 229
• Page End: 242
• Publication Date: 2016-03
• Subjects: DNA damage -- epigenetics -- lipidomics -- membrane phospholipids -- poly(ADPribosyl)ation; Biochemical markers -- Periodicals; 610.28
• DOI: 10.2217/bmm.16.3
• Languages: English
• ISSNs: 1752-0363

Abstract

Aim: Establishing by statistical analyses whether the analyses of auto-modified poly(ADP-ribose)polymerase and erythrocyte membrane fatty acid composition (Fat Profile ® ), separately or in tandem, help monitoring the physio-pathology of the cell, and correlate with diseases, if present.Patients & methods: Ninety five subjects were interviewed and analyzed blindly. Blood lymphocytes and erythrocytes were prepared to assay poly(ADP-ribose)polymerase automodification and fatty acid based membrane lipidome, respectively.Results: Poly(ADP-ribose)polymerase automodification levels confirmed their correlation with DNA damage extent, and allowed monitoring disease activity, upon surgical/therapeutic treatment. Membrane lipidome profiles showed lipid unbalance mainly linked to inflammatory states. Statistically both tests were separately significant, and correlated each other within some pathologies.Conclusion: In the laboratory routine, both tests, separately or in tandem, might be a preliminary and helpful step to investigate the occurrence of a given disease. Their combination represents a promising integrated panel for sensible, noninvasive and routine health monitoring.