High-throughput transcriptome and pathogenesis analysis of clinical psoriasis. Issue 2 (May 2020)

• Record Type: Journal Article
• Authors: Yu, Zengyang; Gong, Yu; Cui, Lian; Hu, Yifan; Zhou, Qianqian; Chen, Zeyu; Yu, Yingyuan; Chen, Youdong; Xu, Peng; Zhang, Xilin; Guo, Chunyuan; Shi, Yuling
• Is Part Of: Journal of dermatological science. Volume 98:Issue 2(2020)
• Page Start: 109
• Page End: 118
• Publication Date: 2020-05
• Subjects: DE differentially expressed -- NN healthy control-derived normal skin group -- PN psoriasis lesions-adjacent normal skin group -- PS psoriasis lesion group -- KEGG Kyoto Encyclopedia of Genes and Genomes -- ACSBG1 acyl-CoA synthetase bubblegum family member 1 -- ACOT2 acyl-CoA thioesterase 2 -- CYP27A1 cytochrome P450 family 27 subfamily A member 1 -- ELOVL3 ELOVL fatty acid elongase 3 -- FABP7 fatty acid binding protein 7 -- FADS2 fatty acid desaturase 2 -- PPARG peroxisome proliferator activated receptor gamma -- CFL1 cofilin 1 -- EPHA2 EPH receptor A2 -- HRAS HRas proto-oncogene GTPase -- NGEF neuronal guanine nucleotide exchange factor -- CRY2 cryptochrome circadian regulator 2 -- PER3 period circadian regulator 3 -- NR1D1 nuclear receptor subfamily 1 group D member 1 -- RORC RAR related orphan receptor C -- CLCN5 chloride voltage-gated channel 5 -- ZNF704 zinc finger protein 704; Psoriasis -- High-throughput transcriptome -- PPAR-fatty acid metabolism -- Neural-hormone regulation -- Circadian entrainment; Dermatology -- Periodicals; Skin Diseases -- Periodicals; Dermatologie -- Périodiques; 616.5005
• DOI: 10.1016/j.jdermsci.2020.03.006
• Languages: English
• ISSNs: 0923-1811

Abstract

Highlights: DE_RNAs. PPAR-fatty acids metabolism. Axon guidance. Circadian entrainment. Abstract: Background: Previous psoriasis studies have mostly focused on skin-related immunology, but the exact mechanisms remain elusive. Clinical evidence, such as higher morbidity among obese individuals and emotional factors, indicate that psoriasis is a complex systemic disease. High-throughput transcriptome analysis provides an effective method to comprehensively assess the disease. Objective: The present study is aiming to understand transcriptome changes of clinical psoriasis skins and comprehensively assess the diseases using pathways analysis. Methods: We performed transcriptome sequence of clinical psoriatic samples. Biological pathway analyses were conducted using differentially expressed RNAs, as well as identified competing endogenous RNAs (ceRNAs). qRT-PCR and histological immunofluorescence staining was conducted to verify the differentially expressed RNAs (DE_RNAs) and the three important enriched biological pathways. Results: Numerous DE_RNAs were identified between psoriasis patients and healthy people. Functional analysis indicated PPAR-fatty acids metabolism pathways, neural-hormone regulations, circadian entrainment were the three mostly appeared pathways. For PPAR-fatty acids metabolism pathways, the expression of seven randomly selected genes, including ACSBG1, ACOT2), CYP27A1, ELOVL3, FABP7, FADS2 and PPARG were all significantly decreased in psoriasis lesions. For neural-hormone regulation pathways, the expression of CFL1, EPHA2, HRAS were all significantly upregulated in psoriasis lesions. While the expression of four randomly selected genes from circadian entrainment pathways, including CRY2, PER3, NR1D1 and RORC were all significantly downregulated. Histological immunofluorescence staining of FADS2, EPHA2 and CRY2 were consistent with their genes' expressions. Conclusion: Our results revealed transcriptome changes of psoriasis, and indicated three important pathways involved in psoriasis, including PPAR-fatty acids metabolism pathways, neural-hormone regulations, circadian entrainment.