Long-term observation of antimicrobial susceptibility and molecular characterisation of Campylobacter jejuni isolated in a Japanese general hospital 2000–2017. (September 2019)

• Record Type: Journal Article
• Authors: Yamada, Kageto; Saito, Ryoichi; Muto, Saori; Sasaki, Masakazu; Murakami, Hinako; Aoki, Kotaro; Ishii, Yoshikazu; Tateda, Kazuhiro
• Is Part Of: Journal of global antimicrobial resistance. Volume 18(2019)
• Page Start: 59
• Page End: 63
• Publication Date: 2019-09
• Subjects: Campylobacter jejuni -- Quinolone resistance -- Clonal complex 21 (CC21) -- ST4526; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2019.02.001
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: Antimicrobial susceptibility and sequence type of Campylobacter jejuni isolates were tested. The MIC value of ciprofloxacin gradually rose in Japanese Campylobacter jejuni isolates. One erythromycin-resistant isolate was observed through the entire study period. ST4526 isolates exhibiting quinolone resistance are predominantly spread in Japan. Abstract: Objectives: Campylobacter jejuni ( C. jejuni ) is one of the most common pathogens that causes gastroenteritis. Because there is currently insufficient epidemiological information about the antimicrobial susceptibility and molecular characterisation of clinical isolates of C. jejuni in Japan, this study carried out antimicrobial susceptibility testing and multilocus sequence typing (MLST) of clinical C. jejuni isolates in Tokyo between 2000–2017. Methods: Antimicrobial susceptibility to erythromycin and ciprofloxacin was tested using the broth microdilution method in 430 C. jejuni clinical isolates collected over 18 years, between 2000–2017, at a Tokyo general hospital. To observe the sequence type (ST) evolution, 82 isolates were chosen from three non-consecutive years (16 isolates from 2000, 25 isolates from 2008, and 41 isolates from 2017) and analysed by MLST as a molecular characterisation test. Mutations in the quinolone resistance-determining region of the gyrA and gyrB genes were identified. Results: The rate of resistance to erythromycin was low, but that of ciprofloxacin resistance was 34.9% in 2000–2008 and 41.9% in 2009–2017. The most common clonal complex (CC) identified during the entire period was CC21; ST4526 with ciprofloxacin resistance was highly prevalent in 2017 (6 of 11; 54.5%). Conclusion: The results indicate that the rate of resistance to quinolone has gradually increased. Since ST4526 was not isolated in 2000 and 2008, it is likely that ST4526 is rapidly increasing in Japan.