Changing epidemiology of KPC-producing Klebsiella pneumoniae in Argentina: Emergence of hypermucoviscous ST25 and high-risk clone ST307. (September 2019)

• Record Type: Journal Article
• Authors: Cejas, Daniela; Elena, Alan; Guevara Nuñez, Daiana; Sevillano Platero, Priscila; De Paulis, Adriana; Magariños, Francisco; Alfonso, Claudia; Berger, María Alejandra; Fernández-Canigia, Liliana; Gutkind, Gabriel; Radice, Marcela
• Is Part Of: Journal of global antimicrobial resistance. Volume 18(2019)
• Page Start: 238
• Page End: 242
• Publication Date: 2019-09
• Subjects: KPC-producing Klebsiella pneumoniae -- Sequence type (ST)307, ST25, ST11 and ST392 -- blaKPC-3 -- mgrB inactivation; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2019.06.005
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: Change in the epidemiology of KPC-producing Klebsiella pneumoniae isolates in Argentina. Dissemination of resistant and hypermucoviscous clone: KPC- K. pneumoniae ST25. First detection of K. pneumoniae ST307 and bla KPC-3 in Argentina. mgrB inactivation in colistin resistant KPC- K. pneumoniae isolates. Abstract: Objectives: To assess the epidemiological features of 76 Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-Kp) isolates recovered from three hospitals in Buenos Aires, Argentina, during 2015–2017. Methods: Antimicrobial susceptibilities were determined according to CLSI Clinical and Laboratoy Standards guidelines. Molecular typing of KPC-Kp was performed by pulsed-field gel electrophoresis (PFGE)-Xbal and multilocus sequence typing. Plasmid encoded genes involved in carbapenem, fosfomycin and colistin resistance were detected by polymerase chain reaction (PCR) and sequencing. Also, mgrB inactivation was investigated in those colistin-resistant isolates. Genetic platforms involved in horizontal spread of bla KPC were investigated by PCR mapping. Results: Besides β-lactams, high resistance rates were observed for gentamycin, quinolones and trimethoprim-sulfamethoxazole. KPC-Kp sequence type (ST)258 corresponded to 26% of the isolates, while 42% corresponded to ST25. The other isolates were distributed in a diversity of lineages such as ST11 (10.5%), ST392 (10.5%), ST307, ST13, ST101, ST15 and ST551. bla KPC-2 was detected in 75 of 76 isolates, and one ST307 isolate harboured bla KPC-3 . Tn 4401 was identified as the genetic platform for bla KPC in epidemic lineages such as ST258 and ST307. However, in ST25 and ST392, which are usually not related to bla KPC, a bla KPC -bearing non-Tn 4401 element was identified. Alterations in mgrB were detected in seven of 11 colistin-resistant isolates. Conclusions: Despite previous reports in Argentina, ST258 is no longer the absolute clone among KPC-Kp isolates. In the present study, dissemination of more virulent lineages such as the hypermucoviscous ST25 was detected. The emergence of the high-risk clone ST307 and occurrence of bla KPC-3 was noticed for the first time in this region.