DNA-based stable isotope probing identifies triclosan degraders in nitrification systems under different surfactants. (April 2020)

• Record Type: Journal Article
• Authors: Jia, Jing-Xin; Gao, Jing-Feng; Dai, Hui-Hui; Zhang, Wen-Zhi; Zhang, Da; Wang, Zhi-Qi
• Is Part Of: Bioresource technology. Volume 302(2020)
• Publication Date: 2020-04
• Subjects: Surfactants -- Triclosan -- DNA-based stable isotope probing -- Biodegradation -- Active TCS degraders; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.122815
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Surfactants showed a significant effect on the enhancement of TCS biodegradation. The higher TCS biodegradation efficiency was obtained when adding biosurfactants. DNA-SIP revealed surfactants enhanced the diversity and activity of TCS degraders. Amaricoccus was identified as the major TCS degrader by means of DNA-SIP. DNA-SIP showed Chiayiivirga can only metabolise TCS in the presence of surfactants. Abstract: Three widely-used surfactants, rhamnolipid (RL), sophorolipid (SL) and sodium dodecyl benzene sulfonate (SDBS), were chosen to investigate their effects on the nitrification systems treating step-wised triclosan (TCS). Surfactants had little effects on nitrification. Surfactants could promote the desorption of TCS and enhance the TCS biodegradation in nitrification systems. And TCS biodegradation efficiencies obtained with RL, SL and SDBS were 1.25, 1.23 and 1.14 times higher than the control with 9.0 mg/L TCS, respectively. Illumina MiSeq sequencing showed that Amaricoccus could be resistant to TCS. And Amaricoccus, detected with RL, SL and SDBS, were more abundant than the control. DNA-based stable isotope probing assays revealed Amaricoccus was the major TCS degrader. And the addition of surfactants could obviously increase the diversity of active TCS degraders, especially for biosurfactants. It seems that the addition of surfactants showed positive effects for the nitrification systems treating TCS wastewater.