Structural insights into mammalian mitochondrial translation elongation catalyzed by mtEFG1. (30th June 2020)

• Record Type: Journal Article
• Authors: Kummer, Eva; Ban, Nenad
• Is Part Of: EMBO journal. Volume 39:Number 15(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-06-30
• Subjects: cryo‐EM -- elongation -- mitoribosome -- mtEFG1 -- translation; Molecular biology -- Periodicals; 572.805
• DOI: 10.15252/embj.2020104820
• Languages: English
• ISSNs: 0261-4189

Abstract

Abstract: Mitochondria are eukaryotic organelles of bacterial origin where respiration takes place to produce cellular chemical energy. These reactions are catalyzed by the respiratory chain complexes located in the inner mitochondrial membrane. Notably, key components of the respiratory chain complexes are encoded on the mitochondrial chromosome and their expression relies on a dedicated mitochondrial translation machinery. Defects in the mitochondrial gene expression machinery lead to a variety of diseases in humans mostly affecting tissues with high energy demand such as the nervous system, the heart, or the muscles. The mitochondrial translation system has substantially diverged from its bacterial ancestor, including alterations in the mitoribosomal architecture, multiple changes to the set of translation factors and striking reductions in otherwise conserved tRNA elements. Although a number of structures of mitochondrial ribosomes from different species have been determined, our mechanistic understanding of the mitochondrial translation cycle remains largely unexplored. Here, we present two cryo‐EM reconstructions of human mitochondrial elongation factor G1 bound to the mammalian mitochondrial ribosome at two different steps of the tRNA translocation reaction during translation elongation. Our structures explain the mechanism of tRNA and mRNA translocation on the mitoribosome, the regulation of mtEFG1 activity by the ribosomal GTPase‐associated center, and the basis of decreased susceptibility of mtEFG1 to the commonly used antibiotic fusidic acid. Synopsis: Mitochondrial translation relies on both conserved and mitochondria‐specific features. Cryo‐EM structures provide insights into tRNA translocation during the elongation stage of mitochondrial translation, which is catalyzed by mtEFG1 on the mitoribosome. tRNA‐mRNA translocation is based on conserved large‐scale motions of the small ribosomal subunit head, and interaction of mtEFG1 with the tRNA‐mRNA module. Closure of the ribosomal GTPase‐associated center facilitates translocation of tRNAs by elongation factor G. Increased stability of mtEFG1 switch‐1 rationalizes decreased susceptibility of mitochondrial translation to the antibiotic fusidic acid. Mitochondria‐specific L1 stalk element compensates for loss of flexible L1 stalk rRNA base. Abstract : Cryo‐EM reconstructions of distinct translation elongation steps explain mtEFG1 regulation by the ribosomal GTPase‐associated center and its decreased susceptibility to the commonly used antibiotic fusidic acid.