Potential Effects of Metformin on the Vitality, Invasion, and Migration of Human Vascular Smooth Muscle Cells via Downregulating lncRNA-ATB. (4th January 2022)

• Record Type: Journal Article
• Authors: Jia, Wei; Zhou, Yue; Sun, Lina; Liu, Jianlong; Cheng, Zhiyuan; Zhao, Shuofang
• Other Names: Busardò Francesco Academic Editor.
• Is Part Of: Disease markers. Volume 2022(2022)
• Publication Date: 2022-01-04
• Subjects: Diagnosis -- Periodicals; Biochemical markers -- Periodicals; Pathology -- Periodicals; 616
• DOI: 10.1155/2022/7480199
• Languages: English
• ISSNs: 0278-0240

Abstract

Abstract : Objective . To elucidate the role of metformin in influencing VSMCs via the involvement of lncRNA-ATB. Methods . qRT-PCR was conducted to detect serum levels of lncRNA-ATB and p53 in CHD patients (n = 50 ) and healthy subjects (n = 50 ). Correlation in serum levels of lncRNA-ATB and p53 in CHD patients was assessed by Pearson correlation test. ROC curves were depicted for analyzing the predictive potential of lncRNA-ATB in the occurrence of CHD. After metformin induction in VSMCs overexpressing lncRNA-ATB, relative levels of lncRNA-ATB and p53 were detected. Meanwhile, proliferative, migratory, and invasive abilities in VSMCs were, respectively, examined by CCK-8 and transwell assay. The interaction between lncRNA-ATB and p53 was tested by RIP. In addition, the coregulation of lncRNA-ATB and p53 in cell functions of VSMCs was finally determined. Results . Increased serum level of lncRNA-ATB and decreased p53 level were detected in CHD patients than those of healthy subjects. LncRNA-ATB could interact with p53 and negatively regulate its level. In addition, lncRNA-ATB could serve as a potential biomarker for predicting the occurrence of CHD. The overexpression of lncRNA-ATB triggered viability, migratory, and invasive abilities in VSMCs, and the above trends were abolished by metformin induction. The overexpression of p53 partially abolished the promotive effects of lncRNA-ATB on proliferative, migratory, and invasive abilities in VSMCs. Conclusions . Metformin induction inhibits proliferative, migratory, and invasive abilities in VSMCs by downregulating lncRNA-ATB, which may be related to p53 activation.