Facile and Eco-Friendly Fabrication of Silver Nanoparticles Using Nyctanthes arbor-tristis Leaf Extract to Study Antibiofilm and Anticancer Properties against Candida albicans. (7th January 2022)

• Record Type: Journal Article
• Authors: Rajamohamed, Beema Shafreen; Siddharthan, Seema; Palanivel, Velmurugan; Vinayagam, Mohanavel; Selvaraj, Vijayanand; Subpiramaniyam, Sivakumar; Salmen, Saleh H.; Obaid, Sami Al; Palanivel, Sekar; Subramanian, Senthilkumar
• Other Names: Sharifi Esmaeel Academic Editor.
• Is Part Of: Advances in materials science and engineering. Volume 2022(2022)
• Publication Date: 2022-01-07
• Subjects: Materials science -- Periodicals; Materials science; Periodicals; 620.11
• DOI: 10.1155/2022/2509089
• Languages: English
• ISSNs: 1687-8434

Abstract

Abstract : The synthesis of silver nanoparticles has been gaining more attention in recent years due to their small size and high stability. For this study, silver nanoparticles were biosynthesized from leaf extract of the medicinal plant ( N. arbor-tristis ). Vitally, the shrub with tremendous medicinal usage was diversely observed in South Asia and South East Asia. The synthesized silver nanoparticles were characterized by color visualization, ultraviolet-visible spectrophotometry (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), field emission-scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), and dynamic light scattering (DLS) technique. A sharp peak at 427 nm for biosynthesized nanoparticles was obtained using UV-Vis, which represents surface plasmon resonance. Thus, characterization techniques showed the green synthesis of AgNPs leads to the fabrication of spherical shape particles with a size of 67 nm. Furthermore, AgNPs were subjected to antibiofilm studies against Candida albicans and it was observed that 0.5 μ g mL −1 of AgNPs significantly reduced 50% of biofilm formation. These biosynthesized nanoparticles also showed a considerable reduction in viability of HeLa cells at 0.5 μ g mL −1 . The morphological changes induced by AgNPs were observed by AO/EB staining. The toxic effect of AgNPs was studied using brine shrimp as a model system. Therefore, it is envisaged that further investigation with these AgNPs can replace toxic chemicals, assist in the development of biomedical implants that can prevent biofilm formation, and avoid infections due to C. albicans .