Improvement of early developmental competence of postovulatory‐aged oocytes using metaphase II spindle injection in mice. (27th June 2020) - Ogawa, Tatsuyuki
Abstract: Purpose: Assisted reproductive technology (ART) is a widely applied fertility treatment. However, the developmental competence of aged oocytes from women of a late reproductive age is seriously reduced and the aged oocytes often fail in fertilization even when ART is used. To resolve this problem, we examined usefulness of a new method "the metaphase II spindle transfer (MESI)" as ART using mouse oocytes. Methods: This work was composed of two experiments. First, 24 hours after collection, embryos from oocytes (1‐day‐old oocytes, called postovulatory‐aged oocytes), were observed, after intracytoplasmic sperm injection (ICSI), and it was found that they were not able to reach the blastocyst stage. Next, the metaphase II chromosome‐spindle complexes from 1‐day‐old oocytes were injected into cytoplasts from oocytes just collected, using piezo pulses to generate reconstructed oocytes. This procedure was named metaphase II spindle injection (MESI). Results: After ICSI, embryos from the reconstructed oocytes (32/105), which contained the genes of 1‐day‐old oocytes, were able to develop into the blastocyst stage. The fragmentation rate after ICSI was 28.6%. Thus, the developmental competence of 1‐day‐old oocytes was improved by MESI. Conclusions: The MESI method has the potential to improve the success rate of infertility treatments for women of a late reproductive age. Abstract : The metaphase II chromosome‐spindle complexes from 1‐day‐old oocytes (postovulatory‐aged oocytes 24 hours after collection) were injected into cytoplasts from freshly collected oocytes using piezo pulses to generate reconstructed oocytes (MESI). After ICSI, the embryos from reconstructed oocytes (32/105) could develop into the blastocyst stage. However, the developmental competence of 1‐day‐old oocytes was improved by MESI.