Long-term effect of parental selenium supplementation on the one-carbon metabolism in rainbow trout (Oncorhynchus mykiss) fry exposed to hypoxic stress. Issue 1 (14th January 2022)

• Record Type: Journal Article
• Authors: Wischhusen, Pauline; Heraud, Cécile; Skjærven, Kaja; Kaushik, Sadasivam J.; Fauconneau, Benoit; Antony Jesu Prabhu, Philip; Fontagné-Dicharry, Stéphanie
• Is Part Of: British journal of nutrition. Volume 127:Issue 1(2022)
• Page Start: 23
• Page End: 34
• Publication Date: 2022-01-14
• Subjects: Selenium -- Nutritional programming -- Rainbow trout -- One-carbon metabolism -- Hypoxia; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S000711452100074X
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: This study evaluated how different forms of selenium (S e) supplementation into rainbow trout broodstock diets modified the one-carbon metabolism of the progeny after the beginning of exogenous feeding and followed by hypoxia challenge. The progeny of three groups of rainbow trout broodstock fed either a control diet (S e level: 0·3 µg/g) or a diet supplemented with inorganic sodium selenite (S e level: 0·6 µg/g) or organic hydroxy-selenomethionine (S e level: 0·6 µg/g) was cross-fed with diets of similar S e composition for 11 weeks. Offspring were sampled either before or after being subjected to an acute hypoxic stress (1·7 mg/l dissolved oxygen) for 30 min. In normoxic fry, parental S e supplementation allowed higher glutathione levels compared with fry originating from parents fed the control diet. Parental hydroxy-selenomethionine treatment also increased cysteine and cysteinyl–glycine concentrations in fry. Dietary S e supplementation decreased glutamate–cysteine ligase ( cgl ) mRNA levels. Hydroxy-selenomethionine feeding also lowered the levels of some essential free amino acids in muscle tissue. Supplementation of organic S e to parents and fry reduced betaine-homocysteine S-methyltransferase ( bhmt ) expression in fry. The hypoxic stress decreased whole-body homocysteine, cysteine, cysteinyl-glycine and glutathione levels. Together with the higher mRNA levels of cystathionine beta-synthase ( cbs ), a transsulphuration enzyme, this suggests that under hypoxia, glutathione synthesis through transsulphuration might have been impaired by depletion of a glutathione precursor. In stressed fry, S-adenosylmethionine levels were significantly decreased, but S-adenosylhomocysteine remained stable. Decreased bhmt and adenosylmethionine decarboxylase 1a ( amd1a ) mRNA levels in stressed fry suggest a nutritional programming by parental S e also on methionine metabolism of rainbow trout.