Real‐time augmented reality for delineation of surgical margins during neurosurgery using autofluorescence lifetime contrast. Issue 1 (9th August 2019)

• Record Type: Journal Article
• Authors: Alfonso‐Garcia, Alba; Bec, Julien; Sridharan Weaver, Shamira; Hartl, Brad; Unger, Jakob; Bobinski, Matthew; Lechpammer, Mirna; Girgis, Fady; Boggan, James; Marcu, Laura
• Is Part Of: Journal of biophotonics. Volume 13:Issue 1(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-08-09
• Subjects: brain tumor -- fluorescence lifetime -- imaging -- neurosurgery -- radiation‐induced necrosis; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201900108
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Current clinical brain imaging techniques used for surgical planning of tumor resection lack intraoperative and real‐time feedback; hence surgeons ultimately rely on subjective evaluation to identify tumor areas and margins. We report a fluorescence lifetime imaging (FLIm) instrument (excitation: 355 nm; emission spectral bands: 390/40 nm, 470/28 nm, 542/50 nm and 629/53 nm) that integrates with surgical microscopes to provide real‐time intraoperative augmentation of the surgical field of view with fluorescent derived parameters encoding diagnostic information. We show the functionality and safety features of this instrument during neurosurgical procedures in patients undergoing craniotomy for the resection of brain tumors and/or tissue with radiation damage. We demonstrate in three case studies the ability of this instrument to resolve distinct tissue types and pathology including cortex, white matter, tumor and radiation‐induced necrosis. In particular, two patients with effects of radiation‐induced necrosis exhibited longer fluorescence lifetimes and increased optical redox ratio on the necrotic tissue with respect to non‐affected cortex, and an oligodendroglioma resected from a third patient reported shorter fluorescence lifetime and a decrease in optical redox ratio than the surrounding white matter. These results encourage the use of FLIm as a label‐free and non‐invasive intraoperative tool for neurosurgical guidance. Abstract : An intraoperative fluorescence lifetime imaging (FLIm) instrument is safely used during human brain surgery to resolve distinct tissue types and pathologies, as reported in three case studies. Autofluorescence lifetime contrast provides label‐free biochemical information of distinct brain tissues including cortex, tumors and radiation induced necrosis. The FLIm instrument integrates with surgical microscopes to provide real‐time intraoperative augmentation of the surgical field of view with fluorescent‐derived parameters encoding diagnostic information.