Engineering approaches to control and design the in vitro environment towards the reconstruction of organs. (10th January 2020)

• Record Type: Journal Article
• Authors: Hagiwara, Masaya; Koh, Isabel
• Other Names: Morishita Y. guestEditor.; Kitajima T. guestEditor.; Tagami S. guestEditor.; Takasato M. guestEditor.; Tanaka Y. guestEditor.
• Is Part Of: Development growth and differentiation. Volume 62:Number 3(2020)
• Page Start: 158
• Page End: 166
• Publication Date: 2020-01-10
• Subjects: bioengineering -- cell distribution control -- environmental control -- microfabrication -- microfluidics; Embryology -- Periodicals; Developmental biology -- Periodicals; Growth -- Periodicals; 574.3
• DOI: 10.1111/dgd.12647
• Languages: English
• ISSNs: 0012-1592

Abstract

Abstract: In vitro experimental models pertaining to human cells are considered essential for most biological experiments, such as drug development and analysis of disease mechanisms, because of their genetic consistency and ease for detailed and long‐term analysis. Recent development of organoid cultures, such as intestine, liver, and kidney cultures, greatly promotes the potential of in vitro experiments. However, conventional culture methods that use manual pipetting have limitations in regenerating complex biosystems. Our body autonomously organizes cells to form a specific tissue shape, and the self‐organization process occurs in an extremely systematic manner. In order to emulate this sophisticated process in vitro; first, methodologies for cell culture and organization of in vitro systems need to be updated; second, understanding the self‐organizing system is a crucial issue. In this review, recent advancements in engineering technologies to control the microenvironment during cell culture are introduced. Both static and dynamic control have been developed for decades in engineering fields, and the means by which such technologies can help to elucidate and design a biosystem is discussed. Abstract : In this review, recent advancements in engineering technologies to control the microenvironment during cell culture are introduced. Both static and dynamic control have been developed for decades in engineering fields, and the means by which such technologies can help to elucidate and design a biosystem is discussed.