Impact of Age and Sex on α-Syn (α-Synuclein) Knockdown-Mediated Poststroke Recovery. Issue 10 (October 2020)

• Record Type: Journal Article
• Authors: Chelluboina, Bharath; Kim, Taehee; Mehta, Suresh L.; Kim, Joo-Yong; Bathula, Saivenkateshkomal; Vemuganti, Raghu
• Is Part Of: Stroke. Volume 51:Issue 10(2020)
• Publication Date: 2020-10
• Subjects: brain -- neuroprotection -- reperfusion -- stroke -- synuclein; Cerebrovascular disease -- Periodicals; Cerebral circulation -- Periodicals; 616.81
• DOI: 10.1161/STROKEAHA.120.028978
• Languages: English
• ISSNs: 0039-2499

Abstract

Abstract : Background and Purpose: Increased expression of α-Syn (α-Synuclein) is known to mediate secondary brain damage after stroke. We presently studied if α-Syn knockdown can protect ischemic brain irrespective of sex and age. Methods: Adult and aged male and female mice were subjected to transient middle cerebral artery occlusion. α-Syn small interfering RNA (siRNA) was administered intravenous at 30 minutes or 3 hour reperfusion. Poststroke motor deficits were evaluated between day 1 and 7 and infarct volume was measured at day 7 of reperfusion. Results: α-Syn knockdown significantly decreased poststroke brain damage and improved poststroke motor function recovery in adult and aged mice of both sexes. However, the window of therapeutic opportunity for α-Syn siRNA is very limited. Conclusions: α-Syn plays a critical role in ischemic brain damage and preventing α-Syn protein expression early after stroke minimizes poststroke brain damage leading to better functional outcomes irrespective of age and sex. Abstract : Supplemental Digital Content is available in the text.