Characterization of an arabinogalactan from the fruit hulls of Ficus pumila Linn. and its immunomodulatory effect. (October 2020)

• Record Type: Journal Article
• Authors: Wu, Jianjun; Xu, Yongbin; Zhu, Bo; Liu, Kaohua; Wang, Siqi; Sheng, Yunjie; Wang, Huijun; Shi, Songshan; Zhang, Qiaoyan; Wang, Shunchun; Qin, Luping
• Is Part Of: Journal of functional foods. Volume 73(2020)
• Publication Date: 2020-10
• Subjects: Ficus pumila Linn. fruit hulls -- Arabinogalactan -- Physicochemical properties -- Immunomodulatory effect; AG arabinogalactan -- AG-I type I arabinogalactans -- AG-II type II arabinogalactans -- BCA bicinchoninic acid -- CCK-8 cell counting kit-8 -- ddH2O double-distilled water -- DEAE diethylaminoethyl -- FTIR fourier transform infrared -- FPW the arabinogalactan from the fruit hulls of Ficus pumila Linn -- FPP the crude polysaccharides from the fruit hulls of Ficus pumila Linn -- FPP-W ddH2O fraction of FPP -- HPGPC high-performance gel permeation chromatography -- IL-1β interleukin-1β -- IL-6 interleukin-6 -- LPS lipopolysaccharide -- NO nitric oxide -- OD optical density -- PMAA partially methylated alditol acetate -- SD standard deviation -- SEM scanning electron microscope -- TEM transmission electron microscope -- TFA trifluoroacetic acid -- TLR4 toll-like receptor 4 -- TNF-α tumor necrosis factor α; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104091
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: An arabinogalactan (FPW) was obtained from the fruit hulls of Ficus pumila Linn. The chemical structure of FPW was investigated. FPW exhibited immunomodulatory effect on RAW 264.7 cells. Ficus pumila L. fruit hulls were a potential source of active arabinogalactan. Abstract: Ficus pumila Linn. fruits has a long history of use as a functional food in East Asia. The present study developed a consecutive process for the production of an arabinogalactan (FPW) from the fruit hulls of Ficus pumila Linn.. FPW was identified as a type II arabinogalactan with a 1, 3-β-D-Gal p backbone with O-6 branching to 1, 6-linked Gal side chains or terminal-β-D-Gal p, and has a molecular weight of 156 kDa. FPW was proved be capable of promoting NO production, phagocytic activity, and the secretion of cytokines including TNF- α, IL-1β and IL-6 without inducing substantial cytotoxicity on RAW 264.7 cells, suggesting the potential utilization of FPW as an immunomodulatory agent. The obtained results emphasize Ficus pumila L. fruit hulls as a potentially ideal source of viable and naturally-derived immunomodulatory materials, also providing a method for obtaining the active arabinogalactan from Ficus pumila L. fruit hulls.