A succinyl isoflavone identified in natto promotes anti-ischemic effects in the middle cerebral artery occlusion rats. (October 2020)

• Record Type: Journal Article
• Authors: Bao, Ke; Chen, Tian-Li; Zhang, Sen; Huang, Zi-Zheng; Huang, Yi-Fei; Huang, Zhi-Heng; Zhu, Ya-ying; Wu, Qi-Nan; Duan, Jin-Ao; Zhang, Zhu-Zhe; Wu, Chang-Jian; Ding, Qing-Qing
• Is Part Of: Journal of functional foods. Volume 73(2020)
• Publication Date: 2020-10
• Subjects: Natto -- Anti-ischemia -- Antioxidant -- 6″-O-Succinyldaidzin -- MCAO; MCAO middle cerebral artery occlusion -- MRI magnetic resonance imaging -- Nrf2 nuclear factor E2-related factor 2 -- Keap1 Kelch-like ECH-associated protein1 -- HO-1 heme oxygenase-1 -- ERK extracellular-signal-regulated kinase -- ROS reactive oxygen species -- JNK c-Jun N-terminal kinase -- OGD/R glucose deprivation/ reperfusion -- SD Sprague-Dawley -- T2WI T2 weight -- VEH vehicle group -- CTL control group; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104104
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: 6″-O-Succcinylapigenin can be produced in large amounts by bio-transformation. It is the first time to detect the content of 6″-O-Succcinylapigenin in natto. 6″-O-Succcinylapigenin exhibits the anti-ischemic effect. 6″-O-Succcinylapigenin acts the potential bio-activities of regulating antioxidant. Abstract: 6″-O-Succinyldaidzin is a daidzin derivative in natto with little bioactivity reports. We investigated the anti-ischemic effects and underlying mechanisms of 6″-O-Succinyldaidzin in MCAO model and used MRI to measure the infarct volume, and neurological scores to evaluate the effect of the injury after occlusion for 2 h and reperfusion for 24 h on male rats. Levels of Nrf2, Keap1, HO-1, ERK were measured by immunoblots to study the underlying mechanisms of 6″-O-Succinyldaidzin activity. Further, we confirmed our results in HT-22 cells using immunofluorescence to assay for the expression of Nrf2 and Keap1. 6″-O-Succinyldaidzin reduced both infarct areas and neurological scores of MCAO rats. Levels of ERK, HO-1, and nuclear-Nrf2 were upregulated after the administration of 6″-O-Succinyldaidzin. Immunofluorescence assays indicated that Nrf2 expressed in the nucleus while Keap1 remained in the cytoplasm after treatment with 6″-O-Succinyldaidzin. Our study shows that 6″-O-Succinyldaidzin of natto elicits an anti-ischemic effect and activates Nrf2-related antioxidant.