Degeneration of human orexinergic neurons across Braak stages of Alzheimer's disease: Implication for pathogenesis, sleep dysfunction, and therapy. (December 2021)

• Record Type: Journal Article
• Authors: Larsen, Eva; Oh, Jun Yeop; Mladinov, Mihovil; Lew, Caroline; Li, Song; Neylan, Thomas; Seeley, William W.; Spina, Salvatore; Walsh, Christine M; Grinberg, Lea T.
• Is Part Of: Alzheimer's & dementia. Volume 17(2021)Supplement 3
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12
• Subjects: Alzheimer's disease -- Periodicals; Alzheimer Disease -- Periodicals; Dementia -- Periodicals; Démence; Maladie d'Alzheimer; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 616.83
• DOI: 10.1002/alz.052465
• Languages: English
• ISSNs: 1552-5260

Abstract

Abstract: Background: Sleep disturbances are common precognitive symptoms of Alzheimer's disease (AD). Wake‐promoting orexinergic neurons (Oxe) in the lateral hypothalamic area (LHA) are key regulators of sleep‐wake states. We recently reported a major loss of Oxe in the terminal stages of AD. This was unexpected, because clinical research shows elevated orexin levels in the cerebrospinal fluid (CSF) of AD patients. In line with this finding, suvorexant, an orexin receptor antagonist, was recently the first drug approved for insomnia in patients with mild‐to‐moderate AD. The aim of our current study was to understand the biological basis of these findings and elucidate the still unknown pattern of orexinergic neuron degeneration in the course of AD. Methods: We performed a postmortem study of 15 subjects with different AD stages and controls. Patients with contributing pathologies other than AD were excluded from the study. Using double immunohistochemistry and unbiased stereology, we estimated the number of total neurons, Oxe and tau‐inclusions in the LHA along the Braak stages of AD. Results: Examined participants' mean age was 70± 12 years, 47% female. Along AD progression, there was an increase of tau inclusions in Oxe, with 0.1% of tau‐positive neurons at Braak stage 0 and 23% at stage 6 (Fig. 1). Further, we observed a substantial decrease of ONs from Braak stage 0 to stage 6 (Fig. 2). Along other Braak stages the decrease of orexinergic neuronal number was slower. Conclusion: We provide evidence of tau‐accumulation in Oxe as early as Braak stage 0 and significant orexinergic neuronal loss from Braak 1. This correlates well with clinical data showing that in many patients sleep‐wake disorders precede the cognitive symptoms, which typically emerge with Braak 3‐4 stages. Further, we conclude that elevated orexin levels in the CSF of AD patients may represent a compensatory mechanism for the loss of Oxe in the early and middle disease stages, whereas in the terminal stage the compensatory capacity may be lost. The practical consequence is that during treatment, disease stages should be considered and symptomatic therapy should be adjusted accordingly. Further studies are necessary to develop better targeting / therapeutics for sleep disturbances in AD.