Feasibility of neurochemically profiling mouse embryonic brain and its development in utero using 1H MRS at 14.1 T. (19th August 2019)

• Record Type: Journal Article
• Authors: Lei, Hongxia; Montessuit, Sylvie; Herzig, Sébastien; Martinou, Jean‐Claude
• Is Part Of: NMR in biomedicine. Volume 32:Number 11(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-08-19
• Subjects: embryonic mouse brain -- in utero -- lactate -- mitochondrial pyruvate carrier 1 -- neurochemical profile; Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.4163
• Languages: English
• ISSNs: 0952-3480

Abstract

Abstract : We aimed to evaluate the feasibility of neurochemical profiling of embryonic mouse brain developments in utero and to seek potential in vivo evidence of an energy shift in a mitochondrial pyruvate carrier 1 (MPC1) deficient mouse model. C57BL/6 embryonic mouse brains were studied in utero by anatomical MRI and short echo localized proton ( 1 H) MRS at 14.1 T. Two embryonic stages were studied, the energy shift (e.g., embryonic day 12.5–13, E12.5–13) and close to the birth (E17.5–18). In addition, embryonic brains devoid of MPC1 were studied at E12.5–13. The MRI provided sufficient anatomical contrasts for visualization of embryonic brain. Localized 1 H MRS offered abundant metabolites through the embryonic development from E12.5 and close to the birth, e.g., E17.5 and beyond. The abundant neurochemical information at E12.5 provided metabolic status and processes relating to cellular development at this stage, i.e., the energy shift from glycolysis to oxidative phosphorylation, evidenced by accumulation of lactate in E12.5–13 embryonic brain devoid of MPC1. The further evolution of the neurochemical profile of embryonic brains at E17.5–18 is consistent with cellular and metabolic processes towards the birth. Localized 1 H MRS study of embryonic brain development in utero is feasible, and longitudinal neurochemical profiling of embryonic brains offers valuable insight into early brain development. Abstract : Mouse embryonic brains were studied in utero by anatomical MR images (insets) and localised 1 H MR spectra (a, b) at 14.1T. A high number of metabolites were observed at E12.5‐E13 (a). Substantial spectral differences were observed at E17.5‐E18 (b, with arrows indicating their directions). Quality in vivo 1 H MR spectra provide neurochemical profiling (c) of murine embryonic brain at E12.5 and towards the birth, e.g. E17.5‐18.