Quantitative Particle Uptake by Cells as Analyzed by Different Methods. Issue 14 (13th December 2019)

• Record Type: Journal Article
• Authors: Ashraf, Sumaira; Hassan Said, Alaa; Hartmann, Raimo; Assmann, Marcus‐Alexander; Feliu, Neus; Lenz, Peter; Parak, Wolfgang J.
• Is Part Of: Angewandte Chemie international edition. Volume 59:Issue 14(2020)
• Page Start: 5438
• Page End: 5453
• Publication Date: 2019-12-13
• Subjects: bioanalytics -- cells -- nanoparticles -- particle uptake; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201906303
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: There is a large number of two‐dimensional static in vitro studies about the uptake of colloidal nano‐ and microparticles, which has been published in the last decade. In this Minireview, different methods used for such studies are summarized and critically discussed. Supplementary experimental data allow for a direct comparison of the different techniques. Emphasis is given on how quantitative parameters can be extracted from studies in which different experimental techniques have been used, with the goal of allowing better comparison. Abstract : Time for a recount : In vitro studies on the uptake of colloidal nano‐ and microparticles by cells are summarized and critically discussed. Supplementary experimental data allow for a direct comparison of the different techniques. Emphasis is given on how quantitative parameters can be extracted from studies in which different experimental techniques have been used.