Biomineral Precursor Formation Is Initiated by Transporting Calcium and Phosphorus Clusters from the Endoplasmic Reticulum to Mitochondria. Issue 8 (2nd March 2020)

• Record Type: Journal Article
• Authors: Tang, Cuizhu; Wei, Yan; Gu, Lin; Zhang, Qinghua; Li, Mei; Yuan, Guohua; He, Yuan; Huang, Li; Liu, Yan; Zhang, Yufeng
• Is Part Of: Advanced science. Volume 7:Issue 8(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-03-02
• Subjects: biomineralization -- black phosphorus -- endoplasmic reticulum -- mineral precursors -- mitochondrion; Science -- Periodicals; 505
• DOI: 10.1002/advs.201902536
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Mineral granules in the mitochondria of bone‐forming cells are thought to be the origin of biomineral precursors, which are transported to extracellular matrices to initiate cell‐mediated biomineralization. However, no evidence has revealed how mitochondrial granules form. This study indicates that mitochondrial granules are initiated by transporting calcium and phosphorus clusters from the endoplasmic reticulum (ER) to mitochondria based on detailed observations of the continuous process of mouse parietal bone development as well as in vitro biomineralization in bone‐forming cells. Nanosized biomineral precursors (≈30 nm in diameter), which originate from mitochondrial granules, initiate intrafibrillar mineralization of collagen as early as embryonic day 14.5. Both in vivo and in vitro studies further reveal that formation of mitochondrial granules is induced by the ER. Elevated levels of intracellular calcium or phosphate ions, which can be induced by treatment with ionomycin and black phosphorus, respectively, accelerate formation of the calcium and phosphorus clusters on ER membranes and ultimately promote biomineralization. These findings provide a novel insight into biomineralization and bone formation. Abstract : The origin of mineral granules in the mitochondria, which are considered as biomineral precursors involved in the biomineralization process, has not been revealed. This study indicates that mitochondrial granules are initiated by transporting calcium and phosphorus clusters from the endoplasmic reticulum to mitochondria. Elucidation of the natural development of biomineral precursors is essential for a better understanding of biomineralization.