Integrating Time‐Resolved Imaging Information by Single‐Luminophore Dual Thermally Activated Delayed Fluorescence. (26th August 2020)

• Record Type: Journal Article
• Authors: Luo, Mengkai; Li, Xuping; Ding, Longjiang; Baryshnikov, Gleb; Shen, Shen; Zhu, Mingjie; Zhou, Lulu; Zhang, Man; Lu, Jianjun; Ågren, Hans; Wang, Xu‐dong; Zhu, Liangliang
• Is Part Of: Angewandte Chemie. Volume 132:Number 39(2020)
• Page Start: 17166
• Page End: 17173
• Publication Date: 2020-08-26
• Subjects: aggregated state -- anti-Kasha's rule -- bioimaging -- dual emissions -- thermally activated delayed fluorescence; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202009077
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: The fact that the lifetime of photoluminescence is often difficult to access because of the weakness of the emission signals, seriously limits the possibility to gain local bioimaging information in time‐resolved luminescence probing. We aim to provide a solution to this problem by creating a general photophysical strategy based on the use of molecular probes designed for single‐luminophore dual thermally activated delayed fluorescence (TADF). The structural and conformational design makes the dual TADF strong in both diluted solution and in an aggregated state, thereby reducing sensitivity to oxygen quenching and enabling a unique dual‐channel time‐resolved imaging capability. As the two TADF signals show mutual complementarity during probing, a dual‐channel means that lifetime mapping is established to reduce the time‐resolved imaging distortion by 30–40 %. Consequently, the leading intracellular local imaging information is serialized and integrated, which allows comparison to any single time‐resolved signal, and leads to a significant improvement of the probing capacity. Abstract : Luminogens for a superior time‐resolved (TR) imaging effect with channel selectivity were achieved by engineering dual thermally activated delayed fluorescence (TADF) from anti‐Kasha/Kasha emission pathways. Intracellular local imaging information that is serialized and integrated was observed by calibrating the two TR signals. The technology is pertinent to precision medicine as TR signals are typically difficult to access in weak emission areas.