The MinD homolog FlhG regulates the synthesis of the single polar flagellum of Vibrio alginolyticus. Issue 1 (17th July 2015)

• Record Type: Journal Article
• Authors: Ono, Hiroki; Takashima, Akari; Hirata, Hikaru; Homma, Michio; Kojima, Seiji
• Is Part Of: Molecular microbiology. Volume 98:Issue 1(2015)
• Page Start: 130
• Page End: 141
• Publication Date: 2015-07-17
• Subjects: Molecular microbiology -- Periodicals; 572.829
• DOI: 10.1111/mmi.13109
• Languages: English
• ISSNs: 0950-382X

Abstract

Summary: FlhG, a MinD homolog and an ATPase, is known to mediate the formation of the single polar flagellum of V ibrio alginolyticus together with FlhF. FlhG and FlhF work antagonistically, with FlhF promoting flagellar assembly and FlhG inhibiting it. Here, we demonstrate that purified FlhG exhibits a low basal ATPase activity. As with MinD, the basal ATPase activity of FlhG can be activated and the D171A residue substitution enhances its ATPase activity sevenfold. FlhG‐D171A localizes strongly at the cell pole and severely inhibits motility and flagellation, whereas the FlhG K31A and K36Q mutants, which are defective in ATP binding, do not localize to the poles, cannot complement a flhG mutant and lead to hyperflagellation. A strong polar localization of FlhF is observed with the K36Q mutant FlhG but not with the wild‐type or D171A mutant FlhG. Unexpectedly, an Ala substitution at the catalytic residue (D60A), which abolishes ATPase activity but still allows ATP binding, only slightly affects FlhG functions. These results suggest that the ATP‐dependent polar localization of FlhG is crucial for its ability to downregulate the number of polar flagella. We speculate that ATP hydrolysis by FlhG is required for the fine tuning of the regulation. Abstract : This paper describes a molecular mechanism of how the MinD homolog FlhG regulates biogenesis of the single polar flagellum of marine bacterium Vibrio alginolyticus. Results reveal that polar localization of FlhF is critical for flagellation and that FlhG negatively regulates FlhF polar localization in an ATP‐dependent manner. These findings provide insight into the spatial and numerical control of the flagellar assembly and reveal parallels between control of flagellar assembly and MinCDE‐mediated control of septation. (74 words)