Cellulose membrane modified with LED209 as an antibacterial and anti-adhesion material. (15th January 2021)

• Record Type: Journal Article
• Authors: Zhou, Zongbao; Dong, Zixuan; Wang, Lei; Song, Rijian; Mei, Naibin; Chen, Tao; Luo, Lei; Ding, Qiang; Wang, Xiaoying; Tang, Shunqing
• Is Part Of: Carbohydrate polymers. Volume 252(2021)
• Publication Date: 2021-01-15
• Subjects: Surface modification -- LED209 -- cellulose membrane -- click reaction -- anti-adhesion activity -- EHEC; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117138
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical Abstract: Highlights: LED209 attached on cellulose membrane as the novel anti-adhesion surface. LED209-functionalized cellulose membrane performed good anti-adhesion properties. The modified membrane repressed the expression of bacterial virulence genes. The modified membrane indicated no cytotoxicity to mammalian cells. Abstract: Bacterial adhesion infection caused by medical materials in clinical application has become a serious threat, and it urgently needs new strategies to deal with these clinical challenges. In this work, LED209, a highly selective histidine sensor kinase inhibitor of Gram-negative bacteria, was covalently attached on cellulose membrane (CM) via click reaction. The data of contact angle measurements, ATR-FTIR and X-ray photoelectron spectroscopy confirmed the successful synthesis of LED-CM. In addition, the results of antibacterial activity of the membranes shown that LED-CM exhibited excellent anti-adhesion ability to Enterohemorrhagic Escherichia coli (EHEC), and significantly reduced the formation of bacterial biofilm. Importantly, LED-CM was able to repress the expression of virulence genes in EHEC. Furthermore, LED209-functionalized cellulose membrane indicated no cytotoxicity to mammalian cells. Hence, our present work demonstrated that CM modified with LED209 possessed markedly anti-adhesion activity against EHEC, which offered a potent antimicrobial material for combating bacterial infections.