Analysis of celluloses, plant holocelluloses, and wood pulps by size-exclusion chromatography/multi-angle laser-light scattering. (1st January 2021)

• Record Type: Journal Article
• Authors: Ono, Yuko; Isogai, Akira
• Is Part Of: Carbohydrate polymers. Volume 251(2021)
• Publication Date: 2021-01-01
• Subjects: cellulose -- holocellulose -- LiCl/N, N-dimethylacetamide -- SEC/MALLS/RI -- molar mass distribution; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117045
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: EDA pretreatment enables dissolution of tunicate and algal celluloses in LiCl/DMAc. Plant holocelluloses also dissolve in LiCl/DMAc by EDA pretreatment. Molar mass distributions of celluloses/holocelluloses by SEC/MALLS/RI analysis. SEC/MALLS analysis reveals presence of branched cellulose structures in softwood. Accurate cellulose d n /d c value must be determined by on-line SEC/MALLS/RI analysis. Abstract: Size-exclusion chromatography with multi-angle laser-light scattering and refractive index detection (SEC/MALLS/RI) provides the number- and weight-average molar masses, molar mass distributions, conformations, and linear/branched structures of polymers. In the case of pure celluloses including highly crystalline tunicate and alga celluloses, and hemicellulose-rich plant holocelluloses, soaking in ethylene diamine (EDA) and subsequent solvent exchange to N, N -dimethylacetamide (DMAc) though methanol is effective for complete dissolution in ∼8% (w/w) LiCl/DMAc. SEC/MALLS/RI analysis can, therefore, be applied to pure celluloses, chemical wood pulps, and plant holocelluloses after dissolution in ∼8% (w/w) LiCl/DMAc, dilution to 1% (w/v) LiCl/DMAc and membrane filtration. All pure celluloses and the high-molar-mass cellulose fractions of hardwood and grass holocelluloses have linear and random-coil conformations and various average molar masses and molar mass distributions depending on the cellulose and holocellulose resources. In contrast, Japanese cedar (i.e., softwood) holocellulose and softwood bleached kraft pulp have alkali-stable cellulose/glucomannan branched structures in the high-molar-mass fractions.