Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis. (1st January 2021)

• Record Type: Journal Article
• Authors: Zhang, Hua; Zou, Pan; Zhao, Haitian; Qiu, Junqiang; Regenstein, Joe Mac; Yang, Xin
• Is Part Of: Carbohydrate polymers. Volume 251(2021)
• Publication Date: 2021-01-01
• Subjects: ABTS 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonate -- FTIR transform infrared spectroscopy -- GC–MS gas chromatography–mass spectroscopy -- HPGPC high performance gel permeation chromatography -- MW molecular weight -- NMR nuclear magnetic resonance -- PKP Pinus koraiensis polysaccharides -- PKP-E polysaccharide fraction of PKP using 80 % ethanol -- PKP-E-1−1, -1−2, -2−1, and -2−2 polysaccharides isolated from the PKP-E using DEAE-52 and Sephadex G-100 columns -- ROS reactive oxygen species -- TCA trichloroacetic acid -- TFA trifluoroacetic acid; Pinecones -- Pinus koraiensis -- PKP-E -- Polysaccharide -- Structure characterization; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117078
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Four novel homogeneous polysaccharides with antioxidant activities were isolated from PKP-E. The structural features of the 4 fractions were characterized using multiple analysis methods. The backbone of 4 fractions contained →2, 6)-β-d -Man-(1→, and →6)-α-d -Gal p A-(1→), analogous to pectin. PKP-E-2-1 was the most stable and active fraction by hydroxyl and ABTS + scavenging assays. Abstract: The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1−1, -1−2, -2−1, and -2−2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l -rhamnose, l -arabinose, d -mannose, d -glucose, and d -galactose. The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d -Man-(1→ and α-d -Gal p A-(1→), which resembles pectin. Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests. The PKP-E-2−1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS ·+ radicals were 3.0 and 23.6 mg/mL.