Antimicrobial efficacy of nisin-loaded bacterial cellulose nanocrystals against selected meat spoilage lactic acid bacteria. (1st January 2021)

• Record Type: Journal Article
• Authors: Gedarawatte, Shamika T.G.; Ravensdale, Joshua T.; Al-Salami, Hani; Dykes, Gary A.; Coorey, Ranil
• Is Part Of: Carbohydrate polymers. Volume 251(2021)
• Publication Date: 2021-01-01
• Subjects: BC bacterial cellulose -- BCNs bacterial cellulose nanocrystals -- LAB lactic acid bacteria -- MRS Man, Rogosa and Sharpe -- NBCNs nisin-loaded bacterial cellulose nanocrystals -- PBS phosphate-buffered saline; Bacterial cellulose -- Nisin -- Nanocrystals -- Antimicrobial activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117096
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Nisin-loaded BCNs were produced by the complexation method. Antimicrobial and physicochemical properties of nisin-loaded BCNs were evaluated. BCNs encapsulated with nisin (≥2 mg/ml) exhibited antimicrobial activity. Nisin-loaded BCNs may be used as reinforcing agent in active food packaging. Abstract: This study aimed to explore the potential of bacterial cellulose nanocrystals (BCNs) loaded with nisin against selected meat spoilage lactic acid bacteria (LAB) in vitro . BCNs were produced by H2 SO4 hydrolysis, and nisin-loaded BCNs were produced through the complexation method. All nanocrystals were assessed for their zeta-potential, encapsulation efficiency and antimicrobial activity. Different nisin concentrations were tested and the most effective nanocrystals were further characterised. BCNs had an average zeta-potential of - 43 mV and all nisin-loaded BCNs produced with 5 mg/ml BCNs suspension had zeta-potential values ≥− 30 mV. The encapsulation efficiency of nisin varied from 80.5 to 93.3 % and crystallinity of BCNs was not influenced by nisin encapsulation. Microbial inactivation was achieved by BCN loaded with 2.0 and 2.5 mg/ml nisin. Therefore, nisin-loaded BCNs may be used as antimicrobial agents in active food packaging.