Structural elucidation of a branch-on-branch β-glucan from Hericium erinaceus with A HPAEC-PAD-MS system. (1st January 2021)

• Record Type: Journal Article
• Authors: Xie, Bingying; Yi, Lin; Zhu, Yiting; Chang, Xiaoming; Hao, Jie; Pang, Li; Ouyang, Yilan; Yuan, Sheng; Zhang, Zhenqing
• Is Part Of: Carbohydrate polymers. Volume 251(2021)
• Publication Date: 2021-01-01
• Subjects: Hericium erinaceus -- β-Glucan -- Sequence analysis -- HPACE-PAD -- MS/MS; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117080
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A 1−6 glucanase was firstly used to digest a β-glucan from H. erinaceus . The oligosaccharides in the digested products were analyzed with HPAEC-MS. The sequences of enzyme resistant oligosaccharides were elucidated with MS/MS. A branch-on-branch structure of the β-glucan was estimated. Abstract: A water-soluble β-glucan from fruiting body of Hericium erinaceus was obtained after water extraction, purification and fractionation. Analyses of monosaccharide composition, molecular weight and linkage mode demonstrated that it is a β-glucan with 1→3 and 1→6 linkage modes and a molecular weight of 13.3 kDa. An endo-1, 6-β-d -glucanase was used to digest the β-glucan and the digested products over time were analyzed with a HPAEC-PAD-MS platform. The linkage mode of each glycosidic bond in the digested oligosaccharides were confirmed with MS/MS. At the end of digestion, enzyme resistant oligosaccharides were observed as several 1→6 linked glucoses with one or two 1→3 linked glucose residues. All these domains are more like constructional pieces from a branch-on-branch glucan, in which multiple 1→6 linked glucan chains are hooked through one or two 1→3 linked glucose residues. Averagely, there is a 1→3 linkage per six 1→6 linked glucoses in this branch-on-branch β-glucan.