Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells. (March 2015)

• Record Type: Journal Article
• Authors: de Girolamo, Laura; Niada, Stefania; Arrigoni, Elena; Di Giancamillo, Alessia; Domeneghini, Cinzia; Dadsetan, Mahrokh; Yaszemski, Michael J; Gastaldi, Dario; Vena, Pasquale; Taffetani, Matteo; Zerbi, Alberto; Sansone, Valerio; Peretti, Giuseppe M; Brini, Anna T
• Is Part Of: Regenerative medicine. Volume 10:Number 2(2015)
• Page Start: 135
• Page End: 151
• Publication Date: 2015-03
• Subjects: adipose-derived stem cells -- bone -- cartilage -- oligo(polyethylene glycol)fumarate hydrogel -- osteochondral defects; Cellular therapy -- Periodicals; Stem cells -- Transplantation -- Periodicals; Degeneration (Pathology) -- Treatment -- Periodicals; Regeneration (Biology) -- Periodicals; 611.018
• DOI: 10.2217/rme.14.77
• Languages: English
• ISSNs: 1746-0751

Abstract

Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months.Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs.Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression.Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.