Flaviflexus ciconiae sp. nov., isolated from the faeces of the oriental stork, Ciconia boyciana. Issue 10 (4th October 2020)

• Record Type: Journal Article
• Authors: Lee, Jae-Yun; Kang, Woorim; Kim, Pil Soo; Lee, So-Yeon; Shin, Na-Ri; Sung, Hojun; Lee, June-Young; Yun, Ji-Hyun; Jeong, Yun-Seok; Han, Jeong Eun; Jung, Mi-Ja; Hyun, Dong-Wook; Kim, Hyun Sik; Tak, Euon Jung; Bae, Jin-Woo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 10(2020)
• Page Start: 5439
• Page End: 5444
• Publication Date: 2020-10-04
• Subjects: Actinobacteria -- Ciconia boyciana -- Flaviflexus -- Flaviflexus ciconiae sp. nov. -- genome -- gut microbiota; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004435
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48 T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea. Optimal growth of strain H23T48 T was observed at 30–37 °C, pH 8 and with 3 % (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48 T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2 T and Flaviflexus huanghaiensis H5 T, respectively. Strain H23T48 T possessed MK-9(H4 ) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω 9 c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids. The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids. The amino acid composition of the cell-wall peptidoglycan was l- alanine, l- lysine, d- glutamic acid, l- aspartic acid and glycine. The genomic G+C content of strain H23T48 T is 59.5 mol% and the average nucleotide identity value between H23T48 T and F. salsibiostraticola KCT C33148 T (=EBR4-1-2 T ) is 75.5 %. Based on the obtained data, strain H23T48 T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp. nov. is proposed. The type strain is H23T48 T (=KCTC 49253 T =JCM 33282 T ).