Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis. Issue 10 (26th October 2020)

• Record Type: Journal Article
• Authors: Benndorf, René; Martin, Karin; Küfner, Michelle; de Beer, Z. Wilhelm; Vollmers, John; Kaster, Anne-Kristin; Beemelmanns, Christine
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 10(2020)
• Page Start: 5255
• Page End: 5262
• Publication Date: 2020-10-26
• Subjects: Actinomadura -- Macrotermes natalensis -- new natural products -- termite gut; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004403
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : The taxonomic positions of two novel aerobic, Gram-positive actinobacteria, designated strains RB29 T and RB68 T, were determined using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of RB29 T were identified as Actinomadura rayongensis DSM 102126 T (99.2 % similarity) and Actinomadura atramentaria DSM 43919 T (98.7 %), and for strain RB68 T was Actinomadura hibisca DSM 44148 T (98.3 %). Digital DNA–DNA hybridization (dDDH) between RB29 T and its closest phylogenetic neighbours, A. rayongensis DSM 102126 T and A. atramentaria DSM 43919 T, resulted in similarity values of 53.2 % (50.6–55.9 %) and 26.4 % (24.1–28.9 %), respectively. Additionally, the average nucleotide identity (ANI) was 93.2 % (94.0 %) for A. rayongensis DSM 102126 T and 82.3 % (78.9 %) for A. atramentaria DSM 43919 T . dDDH analysis between strain RB68 T and A. hibisca DSM 44148 T gave a similarity value of 24.5 % (22.2–27.0 %). Both strains, RB29 T and RB68 T, revealed morphological characteristics and chemotaxonomic features typical for the genus Actinomadura, such as the presence of meso -diaminopimelic acid in the cell wall, galactose and glucose as major sugar components within whole-cell hydrolysates and the absence of mycolic acids. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. Predominant menaquinones were MK-9(H6 ) and MK-9(H8 ) for RB29 T and MK-9(H4 ) and MK-9(H6 ) for RB68 T . The main fatty acids were identified as 10-methyloctadecanoic acid (10-methyl C18:0 ), 14-methylpentadecanoic acid (iso-C16:0 ), hexadecanoic acid (C16:0 ) and cis -9-octadecanoic acid (C18 : 1 ω9 c ). Here, we propose two novel species of the genus Actinomadura : Actinomadura rubteroloni sp. nov. with the type strain RB29 T (=CCUG 72668 T =NRRL B-65537 T ) and Actinomadura macrotermitis sp. nov. with the type strain RB68 T (=CCUG 72669 T =NRRL B-65538 T ).