An immunosensor detects carcinoembryonic antigen by a double reduction strategy based on polyphenylamine as a sacrifice reducing agent. (7th February 2020)

• Record Type: Journal Article
• Authors: Zhao, Chulei; Ma, Chaoyun; Wu, Mei; Li, Wenjun; Song, Yiju; Yang, Yuxiao; Yin, Bincheng; Hong, Chenglin; Qiao, Xiuwen
• Is Part Of: International journal of hydrogen energy. Volume 45:Number 7(2020)
• Page Start: 5055
• Page End: 5066
• Publication Date: 2020-02-07
• Subjects: Immunosensor -- Sacrificial reducing agent -- MnO2; Hydrogen as fuel -- Periodicals; Hydrogène (Combustible) -- Périodiques; Hydrogen as fuel; Periodicals; 665.81
• DOI: 10.1016/j.ijhydene.2019.12.041
• Languages: English
• ISSNs: 0360-3199

Abstract

Abstract: In this paper, a novel Ag NPs-PANI/MnO2 tag was synthesized for the first time, and the electrochemical immunoassay for carcinoembryonic antigen (CEA) was achieved. In this experiment, gold nanoparticles (Au NPs) were used as an electrochemical sensing platform to increase conductivity and improve electrode surface capture antibody (Ab1 ). We use PANI as a green sacrificial agent reduces Ag NPs and MnO2, while improving the conductivity and electrochemical stability of the immunosensor. MnO2 and Ag NPs effectively raise the sensitivity of the sensor by catalytically reducing hydrogen peroxide (H2 O2 ) into water and oxygen to achieve a double amplified sensing signal. It is found that the surface plasmon resonance of Ag NPs has an interesting SERS characteristic structure, and the equivalent circuit simulation of the electrode assembly process results in an error of less than 10%, indicating that the electrochemical immunosensor is successfully prepared. Under optimal conditions, the electrochemical immunosensor has a linear range of 0.0005–80 ng/mL and a detection limit of 0.17 pg/mL (S/N = 3). The immunosensor is expected to be used in clinical laboratories to detect CEA and other tumor markers in human plasma for precancerous screening or surveillance. Highlights: PANI as a green environmentally friendly sacrificial agent to reduce Ag NPs and MnO2 . Ag NPs and MnO2 reduce H2 O2 to make double amplification of the sensing signal. A new type of electrochemical immunosensor has been designed to detect CEA with certain clinical value.