Rapid Screening and Characterization of Triterpene Saponins from the Root of Phytolacca acinosa Roxb by High-Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry. (24th February 2021)

• Record Type: Journal Article
• Authors: Ling, Yun; Zhu, Yue; Gan, Qiao; Li, Guilan; Luo, Zefan; Pan, Yali; Zhao, Lian; Lei, Zhineng; He, Xin; Zeng, Ming; Liu, Wenbo
• Is Part Of: Journal of chromatographic science. Volume 60:Number 1(2022)
• Page Start: 16
• Page End: 25
• Publication Date: 2021-02-24
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1093/chromsci/bmab017
• Languages: English
• ISSNs: 0021-9665

Abstract

Abstract: Triterpene saponins (TSs) are important bioactive constituents with structural diversity widely distributed in many plants. The root of Phytolacca acinosa Roxb (RPa) has been used as a traditional Chinese medicine. However, TSs as the main active ingredients in RPa have not been fully characterized. Here, we profiled TSs from RPa by high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS). We tentatively identified 29 TSs, including 13 that had not been reported previously from this plant. This study indicates that HPLC-ESI-QTOF-MS/MS is an effective and rapid method for the characterization of complicated TSs in herbal extracts.