A gene regulatory network for tiller development mediated by Tin8 in maize. (28th August 2021)

• Record Type: Journal Article
• Authors: Lin, Zhelong; Zhou, Leina; Zhong, Shuyang; Fang, Xiaojian; Liu, Hangqin; Li, Yan; Zhu, Can; Liu, Jiacheng; Lin, Zhongwei
• Editors: Murray, James
• Is Part Of: Journal of experimental botany. Volume 73:Number 1(2022)
• Page Start: 110
• Page End: 122
• Publication Date: 2021-08-28
• Subjects: Domestication -- gene regulatory network -- maize -- Tb1 -- tiller number -- Tin8; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erab399
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract : The maize Tin8 gene controls a complex gene regulatory network for tiller development through an interaction with Tb1 . Abstract: The complex gene regulatory network underlying tiller development in maize remains largely unknown. Here we identified two major quantitative trait loci for tiller number, Tin8 on chromosome 8 and the previously known Tb1 on chromosome 1, in a population derived from a teosinte–maize cross. Map-based cloning and association mapping revealed that Tin8, corresponding to Zcn8 encoding a phosphatidylethanolamine-binding-related kinase, is down-regulated in transcription, which results in decreased tiller number. A strong interaction between Tin8 and the key gen Tb1 was detected for tiller number. Further RNA-seq analysis showed that the expression of 13 genes related to tiller development was controlled by Tin8 . Our results support the existence of a complex gene regulatory network for the outgrowth of the tiller bud in maize, in which Zcn8 controls 13 tiller-related genes, including four genes for hormonal responses. In particular, Zcn8 represses Gt1, D14, and Tru1 through the interaction with Tb1 .