Identification and characterization of class 1 integrons among multidrug-resistant uropathogenic Escherichia coli strains in Mexico. (January 2022)

• Record Type: Journal Article
• Authors: González-Villalobos, Edgar; Ribas-Aparicio, Rosa María; Belmont-Monroy, Laura; Aparicio-Ozores, Gerardo; Manjarrez-Hernández, H. Ángel; Gavilanes-Parra, Sandra; Balcázar, José Luis; Molina-López, José
• Is Part Of: Microbial pathogenesis. Volume 162(2022)
• Publication Date: 2022-01
• Subjects: Multidrug resistance -- Class 1 integron -- Uropathogenic E. coli (UPEC); Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2021.105348
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: This study aimed to identify and characterize integrons among multidrug-resistant (MDR) uropathogenic Escherichia coli (UPEC) from outpatients in Mexico City, Mexico. PCR assays were used to screen for the presence of class 1, 2 and 3 integrons, whose PCR products were sequenced to identify the inserted gene cassettes within the variable regions. Out of 83 tested strains, 53 (63.9%) were positive for the presence of class 1 integrons, whereas no integrons were detected in the remaining strains, regardless of their classes. Most of the strains carrying the intI1 gene belonged to the extraintestinal B2 (41.5%) and commensal A (32.1%) phylogroups, and to a lesser extent, the extraintestinal D (20.8%) and commensal B1 (5.7%) phylogroups. Moreover, 8 different gene cassette arrangements were detected, with dfrA17 and aadA 5 being the most common (32.1% of the class 1 integron-positive strains), which confer resistance to trimethoprim/sulfamethoxazole and aminoglycosides, respectively. Our results suggest that class 1 integrons are widely distributed among MDR-UPEC strains in Mexico, which may directly or indirectly contribute to the selection of MDR strains. These findings are important for a better understanding of the factors and mechanisms that promote multidrug resistance among UPEC strains. Highlights: Class 1 integrons were detected in 63.9% of the tested uropathogenic Escherichia coli (UPEC) strains. Class 1 integrons were mainly detected in pathogenic phylogroups B2 and D. Eight different gene cassette arrangements were detected, with dfrA17 and aadA5 being the most common.