The Interplay Between Disordered Regions in RNAs and Proteins Modulates Interactions Within Stress Granules and Processing Bodies. Issue 1 (15th January 2022)

• Record Type: Journal Article
• Authors: Vandelli, Andrea; Cid Samper, Fernando; Torrent Burgas, Marc; Sanchez de Groot, Natalia; Tartaglia, Gian Gaetano
• Is Part Of: Journal of molecular biology. Volume 434:Issue 1(2022)
• Publication Date: 2022-01-15
• Subjects: structural disorder -- interaction networks -- liquid-liquid phase separation -- fuzzy interactions -- RNA and proteins; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2021.167159
• Languages: English
• ISSNs: 0022-2836

Abstract

Graphical abstract: Highlights: Single-stranded elements enriched in stress granules and P-bodies are disordered. RNAs enriched in disorder form many contacts with RNAs. Proteins enriched in disorder bind to disordered RNAs. Abstract: Condensation, or liquid-like phase separation, is a phenomenon indispensable for the spatiotemporal regulation of molecules within the cell. Recent studies indicate that the composition and molecular organization of phase-separated organelles such as Stress Granules (SGs) and Processing Bodies (PBs) are highly variable and dynamic. A dense contact network involving both RNAs and proteins controls the formation of SGs and PBs and an intricate molecular architecture, at present poorly understood, guarantees that these assemblies sense and adapt to different stresses and environmental changes. Here, we investigated the physico-chemical properties of SGs and PBs components and studied the architecture of their interaction networks. We found that proteins and RNAs establishing the largest amount of contacts in SGs and PBs have distinct properties and intrinsic disorder is enriched in all protein-RNA, protein-protein and RNA-RNA interaction networks. The increase of disorder in proteins is accompanied by an enrichment in single-stranded regions of RNA binding partners. Our results suggest that SGs and PBs quickly assemble and disassemble through dynamic contacts modulated by unfolded domains of their components.