The structures of two polysaccharides from Angelica sinensis and their effects on hepatic insulin resistance through blocking RAGE. (15th March 2022)

• Record Type: Journal Article
• Authors: Liu, Wenjuan; Li, Zezhi; Feng, Caixia; Hu, Shengwei; Yang, Xin; Xiao, Kaimin; Nong, Qiuna; Xiao, Qianhan; Wu, Kehan; Li, Xiao-Qiang; Cao, Wei
• Is Part Of: Carbohydrate polymers. Volume 280(2022)
• Publication Date: 2022-03-15
• Subjects: Angelica sinensis -- Insulin resistance -- Polysaccharide -- Type 2 diabetes -- Structure; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.119001
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: This study found two novel homogeneous polysaccharides from Angelica sinensis, APS-1I and APS-2II, binding to RAGE with a dissociation constant of 2.02 ± 0.2 and 85.92 ± 0.2 μM, respectively. APS-1I is a 17.0 kDa heteropolysaccharide, whose backbone is composed of α-1, 6-Glc p, α-1, 3, 6-Glc p, α-1, 2-Glc p, α-1, 4-Gal p, and α-1, 3-Rha p, and whose two branches contain α-1, 3, 5-Ara f, α-1, 3-Ara f, α-1, 4-Gal p, β-1, 3-Gal p, and β-1, 4-Glc p . APS-2II is a 10.0 kDa linear glucan, that contains α-1, 6-Glc p, α-1, 3-Glc p, α-1, 2-Glc p, and α-T-Glc p . In vitro, APS-1I demonstrated better promotion on glucose absorption and stronger repression on p-IRS-1 (Ser307), p-IRS-2 (Ser731), p-JNK, and p-P38 than APS-2II in insulin resistance (IR)-HepG2 cells. Furthermore, APS-1I treatment couldn't further decrease the inhibition on the phosphorylation of JNK and P38 produced by RAGE siRNA in IR-HepG2 cells. In vivo, APS-1I markedly improved IR and reversed the livers RAGE-JNK/p38-IRS signaling in high-fat-diet and streptozotocin-induced diabetic rats, suggesting that APS-1I could be a potential agent for improving IR in type 2 diabetes. Graphical abstract: Unlabelled Image