Preclinical evaluation of Zanthoxylum piperitum Benn., traditional muscle pain remedy, for joint inflammation. (25th March 2022)

• Record Type: Journal Article
• Authors: Yun, Seung-Won; Seo, Young-Jin; Kwon, Jeong Eun; Park, Dae Won; Lee, Yeong-Geun; Choe, Tae Hwan; Kim, Seul-Ki; Lee, Hak Sung; Kim, Hyunggun; Kang, Se Chan
• Is Part Of: Journal of ethnopharmacology. Volume 286(2022)
• Publication Date: 2022-03-25
• Subjects: BDNF -- Collagen-induced arthritis -- COX-2 -- Phosphor-ERK -- iNOS -- Zanthoxylum piperitum; IκBα inhibitor of NF-κB -- NF-κB nuclear factor κB -- ROS Reactive oxygen species -- MAPKs Mitogen-activated protein kinases -- Ubr4 Ubiquitin Protein Ligase E3 Component N-Recognin 4 -- BDNF Brain-derived neurotrophic factor -- DMARDs disease-modifying anti-rheumatic drugs -- ZPE-LR Zanthoxylum piperitum 90% EtOH extract -- s.c subcutaneous -- RT-PCR reverse transcription polymerase chain reaction -- TBST Tris-buffered saline containing 0.05% Tween-20 -- PBST phosphate-buffered saline containing 0.1% Tween-20 -- ACP annealing control primer -- SEM standard error of the mean; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2021.114921
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Zanthoxylum piperitum has been used as a traditional Asian medicine to treat hypertension, stroke, bruise and muscle pain. It has been known to induce detoxification; affect anti-bacterial, anti-oxidant, and tyrosinase activity; inhibit osteosarcoma proliferation; anti-osteoarthritis inflammation. In this study, we aim to identify the therapeutic effect of Z. piperitum 90% EtOH extract (ZPE-LR) on rheumatoid arthritis. Material and methods: We investigated the anti-rheumatoid arthritis and -immunomodulatory activities of the ZPE-LR in collagen-induced arthritic (CIA) mice, a rheumatoid arthritis animal model. In order to assess the analgesic effects of ZPE-LR in vivo, acetic acid injection, formaldehyde injection, hot plate model was used. The mechanism for anti-inflammatory activity of ZPE-LR was identified with LPS-stimulated Raw 264.7 cells. Results: Pharmacologically, oral administration of ZPE-LR into CIA mice resulted in a significant and dose-dependent decrease in clinical arthritis score and paw swelling compared to untreated negative control. Pathologic examination showed that ZPE-LR prevented morphological change in cartilage and destruction of phalanges in CIA mice. This protective effect was associated with reduced pain, inflammatory mediators such as NO, TNF-α, IL-1β, and IL-6, as well as COX-2 and iNOS expression. Furthermore, reduction of phosphor-ERK and BDNF indicates a novel rheumatoid arthritis-regulating mechanism by ZPE-LR treatment. Conclusions: These data suggest that the administration of ZPE-LR remarkably inhibited CIA progression and might be helpful in suppressing inflammation and pain in rheumatoid arthritis. Graphical abstract: Image 1 Highlights: ZPE-LR have been effectively inhibited on rheumatoid arthritis-induced pain and inflammation. ZPE-LR prevented morphological change in cartilage and destruction of phalanges in CIA mice on pathologic examination.