Behavioural and physiological regulation of protein and carbohydrates in mealworm larvae: A geometric analysis. (January 2022)

• Record Type: Journal Article
• Authors: Rho, Myung Suk; Lee, Kwang Pum
• Is Part Of: Journal of insect physiology. Volume 136(2022)
• Publication Date: 2022-01
• Subjects: Protein -- Carbohydrate -- Feeding behaviour -- Nutrient balancing -- Geometric Framework -- Tenebrio molitor; Insects -- Physiology -- Periodicals; Insectes -- Physiologie -- Périodiques; Insects -- Physiology; Periodicals; 571.157
• DOI: 10.1016/j.jinsphys.2021.104329
• Languages: English
• ISSNs: 0022-1910

Abstract

Graphical abstract: Highlights: Protein-carbohydrate regulation was examined in mealworm larvae. Self-regulated macronutrient intake was slightly carbohydrate-biased. Larvae adopted a nutrient balancing rule characteristic of generalist feeders. Larvae had strong homeostatic regulation of body nutrient growth. Performance was optimized when the intake of protein and carbohydrate was balanced. Abstract: Protein-carbohydrate regulation in the larvae of the mealworm beetle ( Tenebrio molitor L.) was analyzed using the Geometric Framework for nutrition. In this study, the ingestive and post-ingestive responses were measured from T. molitor larvae that were subjected to choice and no-choice experiments. In the choice experiment, T. moitor larvae were simultaneously presented with one of two protein-biased foods (p35:c7 or p28:c5.6) and one of two carbohydrate-biased foods (p7:c35 or p5.6:c28). T. molitor larvae selected protein and carbohydrate in a ratio close to 1:1 over the first 15 days since the start of the experiment (days 0–15), but exhibited preference for carbohydrate-biased food over the next 15 days. The average protein:carbohydrate ratio selected over days 0–30 was 1:1.24. In the no-choice experiment, T. molitor larvae were restricted to one of seven foods with different protein and carbohydrate content (p0:c42, p7:c35, p14:c28, p21:c21, p28:c14, p35:c7, or p42:c0). On the p0:c42 food, consumption was greatly suppressed and no larvae completed their development. Across a range of these foods except p0:c42, T. molitor larvae consistently over-ate the surplus nutrient in the foods and showed a pattern of nutrient balancing similar to that previously described for other nutritional generalists. Despite having consumed substantially different amounts and ratios of macronutrients as larvae, T. molitor pupae in the no-choice food treatments had similar body nutrient composition, suggesting the presence of strong homeostatic regulation for body nutrient growth. Larval survivorship was significantly lower on two extremely imbalanced foods (p7:c35 and p42:0) than on more balanced foods. T. molitor larvae reared on p7:c35 suffered reduced biomass growth and delayed development compared with those on foods with higher protein content.