Effect of BMP6 on the proliferation and apoptosis of chicken chondrocytes induced by thiram. (January 2022)

• Record Type: Journal Article
• Authors: Lu, Yuxiang; Xu, Hengyong; Jiang, Yuru; Li, Dan; Hu, Zhi; Yan, Chaoyang; Yin, Huadong; Li, Diyan; Zhao, Xiaoling; Zhang, Yao; Tian, Yaofu; Zhu, Qing; Wang, Yan
• Is Part Of: Research in veterinary science. Volume 142(2022)
• Page Start: 101
• Page End: 109
• Publication Date: 2022-01
• Subjects: Chicken -- BMP6 -- Tibial dyschondroplasia -- Proliferation -- Apoptosis; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2021.12.002
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: The development of skeleton system is a complex biological process and be regulated by many transcription factors. Previous studies have shown that BMP6 is involved in skeleton development and other cells transforming to chondrocytes, but it is still not known whether do something to tibial dyschondroplasia (TD) broilers chondrocytes. In this study, RT-PCR revealed that the expression level of BMP6 in TD broiler chondrocytes at 7 days age was significantly decreased compared with normal group ( P < 0.05). CCK-8 and EdU assay showed that the proliferation of cells transfected with interference BMP6 was significantly decreased compared with control siRNA, while cell proliferation was significantly increased after overexpression of BMP6. Meanwhile, the proportion of G0 /G1 phase cells was significantly increased and the proportion of G2 /M phase cells was significantly decreased after interference of BMP6 for 48 h in TD chicken chondrocytes ( P < 0.05). In addition, flow cytometry analysis exhibited that interference BMP6 significantly increased apoptosis rate and necrotizing rate of cells. In conclusion, these results suggest that BMP6 plays a positive role in the growth and development of TD broiler chondrocytes. Our findings reveal a new target for TD prevention in broiler chickens. Highlights: The level of BMP6 mRNA in TD broilers induced by thiram was lower than normal group. BMP6 can promote the proliferation of tibial chondrocytes in TD broilers. BMP6 can reduce the apoptosis of tibial chondrocytes in TD broilers.