Application of simultaneous selective pressures slows adaptation. (15th August 2020)

• Record Type: Journal Article
• Authors: Merlo, Lauren M. F.; Sprouffske, Kathleen; Howard, Taylor C.; Gardiner, Kristin L.; Caulin, Aleah F.; Blum, Steven M.; Evans, Perry; Bedalov, Antonio; Sniegowski, Paul D.; Maley, Carlo C.
• Other Names: Thomas Frédéric guestEditor.; Giraudeau Mathieu guestEditor.; Hamede Rodrigo guestEditor.; Roche Benjamin guestEditor.; Ujvari Beata guestEditor.
• Is Part Of: Evolutionary applications. Volume 13:Number 7(2020)
• Page Start: 1615
• Page End: 1625
• Publication Date: 2020-08-15
• Subjects: adaptation -- cancer therapy -- clonal interference -- functional interference -- slowing evolution; Evolution (Biology) -- Periodicals; Genetics -- Periodicals; Natural selection -- Periodicals; Ecology -- Periodicals; 576.8
• DOI: 10.1111/eva.13062
• Languages: English
• ISSNs: 1752-4571

Abstract

Abstract: Beneficial mutations that arise in an evolving asexual population may compete or interact in ways that alter the overall rate of adaptation through mechanisms such as clonal or functional interference. The application of multiple selective pressures simultaneously may allow for a greater number of adaptive mutations, increasing the opportunities for competition between selectively advantageous alterations, and thereby reducing the rate of adaptation. We evolved a strain of Saccharomyces cerevisiae that could not produce its own histidine or uracil for ~500 generations under one or three selective pressures: limitation of the concentration of glucose, histidine, and/or uracil in the media. The rate of adaptation was obtained by measuring evolved relative fitness using competition assays. Populations evolved under a single selective pressure showed a statistically significant increase in fitness on those pressures relative to the ancestral strain, but the populations evolved on all three pressures did not show a statistically significant increase in fitness over the ancestral strain on any single pressure. Simultaneously limiting three essential nutrients for a population of S. cerevisiae effectively slows the rate of evolution on any one of the three selective pressures applied, relative to the single selective pressure cases. We identify possible mechanisms for fitness changes seen between populations evolved on one or three limiting nutrient pressures by high‐throughput sequencing. Adding multiple selective pressures to evolving disease like cancer and infectious diseases could reduce the rate of adaptation and thereby may slow disease progression, prolong drug efficacy and prevent deaths.