A phenothiazine-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in food, live cells and animals. Issue 24 (24th November 2021)

• Record Type: Journal Article
• Authors: Hu, Yaoyun; Shang, Zhuye; Wang, Juan; Hong, Min; Zhang, Run; Meng, Qingtao; Zhang, Zhiqiang
• Is Part Of: Analyst. Volume 146:Issue 24(2021)
• Page Start: 7528
• Page End: 7536
• Publication Date: 2021-11-24
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an01762d
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A phenothiazine-based fluorescent probe features high selectivity and sensitivity, low cytotoxicity and reliability at physiological pH, enabling the detection of H2 S in biosystems and monitoring H2 S produced in the foods spoilage process. Abstract : In this work, a phenothiazine-based fluorescent probe (PR ) has been developed for the selective detection of hydrogen sulfide (H2 S) in biosystems and monitoring H2 S produced in the food spoilage process. The nucleophilic attack of H2 S on the CC double bond of PR via a Michael addition interdicted the ICT process to trigger 34-fold enhancement of the fluorescence emission. PR featured high selectivity and sensitivity (1.8 μM), low cytotoxicity and reliability at physiological pH. "Naked-eye" monitoring of H2 S produced in the food spoilage process using PR was successfully accomplished. The preliminary fluorescence imaging studies showed that PR is suitable for the visualization of exogenous and endogenous H2 S in living cells and live animals. Moreover, PR has been successfully applied to the visualization of H2 S generation in an inflammation model. The results indicated that PR is an effective tool to monitor H2 S production in the fields of biomedicine and food safety.