Absolute quantification of 2‐hydroxyglutarate on tissue by matrix‐assisted laser desorption/ionization mass spectrometry imaging for rapid and precise identification of isocitrate dehydrogenase mutations in human glioma. Issue 12 (4th September 2021)

• Record Type: Journal Article
• Authors: Lan, Chunyan; Li, Hainan; Wang, Lei; Zhang, Jing; Wang, Xiaodong; Zhang, Rumeng; Yuan, Xiaoai; Wu, Taihua; Wu, Jie; Lu, Ming; Ma, Xu
• Is Part Of: International journal of cancer. Volume 149:Issue 12(2021)
• Page Start: 2091
• Page End: 2098
• Publication Date: 2021-09-04
• Subjects: 2‐HG -- absolute quantification -- glioma -- IDH mutations -- MALDI imaging; Cancer -- Periodicals; Cancer -- Prevention -- Periodicals; 616.994
• DOI: 10.1002/ijc.33729
• Languages: English
• ISSNs: 0020-7136

Abstract

Abstract: Isocitrate dehydrogenase (IDH) gene mutations are important predictive molecular markers to guide surgical strategy in brain cancer therapy. Herein, we presented a method using matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) for absolute quantification of 2‐hydroxyglutarate (2‐HG) on tissues to identify IDH mutations and evaluate tumor residue. This analytical method was tested among 34 glioma patients and validated with gold standard clinical technologies. The cut‐off value of 2‐HG was set as 0.81 pmol/μg to identify IDH mutant (IDHmt) gliomas with 100% specificity and sensitivity. In addition, 2‐HG levels and tumor cell density (TCD) showed positive correlation in IDHmt gliomas by this spatial method. This MALDI MSI‐based absolute quantification method has great potentiality for incorporating into surgical workflow in the future.