Cas12a target search and cleavage on force-stretched DNA. Issue 47 (8th September 2021)
- Record Type:
- Journal Article
- Title:
- Cas12a target search and cleavage on force-stretched DNA. Issue 47 (8th September 2021)
- Main Title:
- Cas12a target search and cleavage on force-stretched DNA
- Authors:
- Losito, Marialucrezia
Smith, Quentin M.
Newton, Matthew D.
Cuomo, Maria Emanuela
Rueda, David S. - Abstract:
- Abstract : CRISPR-Cas12a target search and cleavage on force-stretched λ -DNA using optical tweezers. Abstract : Using optical tweezers, we investigate target search and cleavage by CRISPR–Cas12a on force-stretched λ-DNA. Cas12a uses fast, one-dimensional hopping to locate its target. Binding and cleavage occur rapidly and specifically at low forces (≤5 pN), with a 1.8 nm rate-limiting conformational change. Mechanical distortion slows diffusion, increases off-target binding but hinders cleavage.
- Is Part Of:
- Physical chemistry chemical physics. Volume 23:Issue 47(2021)
- Journal:
- Physical chemistry chemical physics
- Issue:
- Volume 23:Issue 47(2021)
- Issue Display:
- Volume 23, Issue 47 (2021)
- Year:
- 2021
- Volume:
- 23
- Issue:
- 47
- Issue Sort Value:
- 2021-0023-0047-0000
- Page Start:
- 26640
- Page End:
- 26644
- Publication Date:
- 2021-09-08
- Subjects:
- Chemistry, Physical and theoretical -- Periodicals
541.3 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cp#!issueid=cp016040&type=current&issnprint=1463-9076 ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/d1cp03408a ↗
- Languages:
- English
- ISSNs:
- 1463-9076
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 6475.306000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 20448.xml