A cysteine-triggered fluorogenic donor base on native chemical ligation for tracking H2S delivery in vivo. Issue 24 (24th November 2021)

• Record Type: Journal Article
• Authors: Li, Li; Lin, Zhenmei; Cheng, Yongfang; Tang, Yaoping; Zhang, Ziqian
• Is Part Of: Analyst. Volume 146:Issue 24(2021)
• Page Start: 7374
• Page End: 7378
• Publication Date: 2021-11-24
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an01809d
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A fluorogenic hydrogen sulfide (H2 S) donor is a fundamental molecular tool used as an exogenous source in biological studies and therapies. Abstract : A hydrogen sulfide (H2 S) donor is a fundamental molecular tool used as an exogenous source in biological studies and therapies. However, finding a controllable and visual fluorescent H2 S donor is difficult. We report a new H2 S donor, HSD560, the H2 S release of which is triggered by cysteine. Importantly, the H2 S generation is accompanied with enhanced green fluorescence, which could be utilized to track H2 S release in cells using microscopy. H2 S release from HSD560 undergoes a non-enzymatic native chemical ligation (NCL) process, which provides an accurate match with activated fluorescence and localization of H2 S in zebrafish.