Shewanella nanhaiensis sp. nov., a marine bacterium isolated from sediment of South China Sea, and emended descriptions of Shewanella woodyi, Shewanella hanedai and Shewanella canadensis. Issue 12 (14th December 2021)

• Record Type: Journal Article
• Authors: Cao, Wen-Rui; Li, Xue; Sun, Yuan-Yuan; Jiang, Ming-Yu; Xu, Xiao-Dong; Li, Ying-Jie
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 12(2021)
• Publication Date: 2021-12-14
• Subjects: Gammaproteobacteria -- Shewanellaceae -- Shewanella nanhaiensis -- polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005152
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, motile, facultative anaerobic and rod-shaped bacterium, designated strain NR704-98 T, was isolated from marine sediment of the northern South China Sea. Cells were positive for oxidase and catalase activity. Growth was observed at 4–30 °C (optimum 20–25 °C), at pH 6–9 (pH 7) and with 0.5–7 % NaCl (2 %). The 16S rRNA gene-based phylogenetic analysis revealed that the nearest phylogenetic neighbours of strain NR704-98 T were Shewanella woodyi MS32 T (97.9 %), Shewanella hanedai 281 T (97.1 %), Shewanella sediminis HAW-EB3 T (96.8 %) and Shewanella canadensis HAW-EB2 T (96.7 %). Based on the results of phylogenomic analysis, the average nucleotide identity and the digital DNA–DNA hybridization values between strain NR704-98 T and the previously mentioned type strains of species of the genus Shewanella were in the range of 74.9–93.1 % and 20.6–51.4 %, respectively. The respiratory quinones were Q-7 and Q-8. The predominant fatty acids (>10 %) of strain NR704-98 T were C16 : 0, summed feature 3 (C16 : 1 ω 7 c and/or C16 : 1 ω 6 c ) and iso-C15 : 0 . Phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminophospholipids and five unidentified lipids were detected in strain NR704-98 T . Based on the phylogenetic and phenotypic characteristics, strain NR704-98 T is considered to represent a novel species of the genus Shewanella, for which the name Shewanella nanhaiensis sp. nov. is proposed. The type strain is NR704-98 T (=KCTC 82799 T =MCCC 1K06091 T ).