Xanthobacter dioxanivorans sp. nov., a 1, 4-dioxane-degrading bacterium. Issue 12 (9th December 2021)

• Record Type: Journal Article
• Authors: Wang, Yingning; Ma, Fang; Yang, Jixian; Guo, Haijuan; Su, Delin
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 12(2021)
• Publication Date: 2021-12-09
• Subjects: 1, 4-dioxane degrader -- Gram-stain-negative -- Xanthobacter dioxanivorans sp. nov.; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005139
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative bacterium, designated as YN2 T, that is capable of degrading 1, 4-dioxane, was isolated from active sludge collected from a wastewater treatment plant in Harbin, PR China. Cells of strain YN2 T were aerobic, motile, pleomorphic rods, mostly twisted, and contained the water-insoluble yellow zeaxanthin dirhamnoside. Strain YN2 T grew at 10–40 °C (optimum, 30 °C), pH 5.0–8.0 (pH 7.0) and with 0–1 % (w/v) NaCl (0.1 %). It also could grow chemolithoautotrophically and fix N2 when no ammonium or nitrate was supplied. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YN2 T belongs to the genus Xanthobacter and shares the highest pairwise identity with Xanthobacter autotrophicus 7c T (98.6 %) and Xanthobacter flavus 301 T (98.4 %). The major respiratory quinone was ubiquinone-10. Chemotaxonomic analysis revealed that the strain possesses C16 : 0, C19 : 0 cyclo ω 8 c and C18 : 1 ω7 c as the major fatty acids. The DNA G+C content was 67.95 mol%. Based on genome sequences, the DNA–DNA hybridization estimate values between strain YN2 T and X. autotrophicus 7c T, X. flavus 301 T and X. tagetidis TagT2C T (the only three species of Xanthobacter with currently available genomes) were 31.70, 31.30 and 28.50 %; average nucleotide identity values were 85.23, 84.84 and 83.59 %; average amino acid identity values were 81.24, 80.23 and 73.57 %. Based on its phylogenetic, phenotypic, and physiological characteristics, strain YN2 T is considered to represent a novel species of the genus Xanthobacter, for which the name Xanthobacter dioxanivorans sp. nov. is proposed. The type strain is YN2 T (=CGMCC 1.19031 T =JCM 34666 T ).